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Registros recuperados: 9
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Characterization of nitrate reductase activity in vitro in Gracilaria caudata J. Agardh (Rhodophyta, Gracilariales) Rev. Bras. Bot.
Chow,Fungyi; Capociama,Fernanda V.; Faria,Renata; Oliveira,Mariana C. de.
The marine red alga Gracilaria caudata J. Agardh has been used in Brazil for agar extraction, mainly in the northeast region of the country. Nitrogen availability is the most important abiotic factor in seawater that limits the growth of seaweeds. The enzyme nitrate reductase (NR) is the key regulatory point in the nitrogen assimilation in photosynthetic organisms. This study describes an in vitro assay, characterizing the enzymatic activity of NR in terms of kinetic constants and stability, its oscillation during the day and glucose effect on NR modulation. Maximal peaks of NR activity were recorded at 20 ºC and pH 8.0. The enzymatic stability in crude extracts stored at 3 ± 1 ºC decreased significantly after 48 hours. Apparent Michaelis-Menten constants...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agarophyte; Gracilaria caudata; Nitrate reductase; Nitrogen metabolism.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042007000100012
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Effects of varying levels of dietary protein and net energy on growth performance, nitrogen balance and faecal characteristics of growing-finishing pigs R. Bras. Zootec.
Zhao,Yumei; Tian,Gang; Chen,Daiwen; Zheng,Ping; Yu,Jie; He,Jun; Mao,Xiangbing; Yu,Bing.
ABSTRACT This experiment was conducted to evaluate the effects of dietary protein and net energy (NE) levels on growth performance, nutrient digestibility, nitrogen metabolism, and faecal microbiota of growing-finishing pigs. Eighteen crossed barrows were randomly allocated into one of three dietary treatments: high protein + high NE diet, low protein + high NE diet, and low protein + low NE diet. The whole experiment lasted 90 days and was divided into three phases (phase I: 25-50 kg; phase II: 50-75 kg; phase III: 75-105 kg). All pigs were individually housed in a metabolism cage and subjected to four-day total faeces and urine collection period at the end of each phase. There was no significant difference in growth performance, nutrient digestibility,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Faecal microbiota; Nitrogen metabolism; Nutrient digestibility; Pigs.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100516
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Leaf proline content and its relation to fruit load and flowering in citrus under field conditions Rev. Bras. Frutic.
Arias-Sibillotte,Mercedes; Borges,Alejandra; Díaz,Pedro; Ferenczi,Alejandra; Severino,Vivian.
Abstract Citrus species grown in temperate zones have sprouting inhibited in winter and this is retaken in spring when they also bloom. The main factor that defines the vegetative or reproductive destiny of the buds is the presence of fruits in development. Low winter temperatures slow plant metabolism, which has been reported as necessary for the expression of the reproductive program. During the winter period of the annual cycle, osmotically active compounds such as proline are increased as part of the cold tolerance response. We investigated whether the presence of developing fruit and the application of exogenous gibberellins affect the proline level in citrus leaves. In an orchard of adult ‘Montenegrina’ mandarin trees, leaf proline contents were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Proline; Flower induction; Nitrogen metabolism; Alternate bearing; Gibberellic acid.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000200201
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Nitrogen metabolism in roots and leaves of green bean plants exposed to different phosphorus doses Phyton
Sánchez,E.; Ávila-Quezada,G.; Gardea,A.A.; Muñoz,E.; Ruiz,J.M.; Romero,L..
The objective of this work was to determine the effect of different P doses on nitrogen assimilation in roots and leaves of green beans plants (Phaseolus vulgaris L. cv. Strike). Phosphorus was applied in the nutrient solution as H3PO4, at the nutrient doses of: P1 = 0.40 mM; P2 = 0.80 mM; P3 = 1.60 mM; P4 = 3.20 mM; P5 = 4.80 mM, and P6 = 6.40 mM P. Our results indicate that both P toxicity and deficiency gave similar responses to N assimilation. Phosphorus and NO3 - interacted on the absorption and translocation processes affecting N assimilation. The deficiency (P1), and toxicity (P6) treatments, diminished root absorption of NO3- in 15% and 36%, respectively, respect to the optimum dose (P3), thus reducing nutrient availability for assimilation. This...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris L.; Nitrogen metabolism; Green bean; Phosphorus; Deficiency; Toxicity.
Ano: 2009 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572009000100003
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Nitrogen translocation in wheat inoculated with Azospirillum and fertilized with nitrogen PAB
RODRIGUES,OSMAR; DIDONET,AGOSTINHO DIRCEU; GOUVEIA,JORGE A.; SOARES,RITA DE CÁSSIA.
The productivity and the translocation of assimilates and nitrogen (N) were compared after inoculation of wheat (Triticum aestivum L., cv. BR-23) seeds with two strains of Azospirillum brasilense (strains 245 and JA 04) under field conditions. The inoculation of wheat seeds was done with a peat inoculant at sowing time. Plant material for evaluations were collected at anthesis and maturity. No differences in grain yield and in the translocation of assimilates resulting from inoculation were detected. Differences were observed in relation to N rates (0, 15, and 60 kg ha-1). N content in the grain increased significantly in the bacteria-inoculated treatments in which N was not added. This increase in N content in the grain with inoculation was probably due...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; Nitrogen fertilization; Nitrogen metabolism; Assimilation; Nitrogen fixing bacteria.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000000700023
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Nutrient digestion, microbial protein synthesis, and blood metabolites of Jersey heifers fed chitosan and whole raw soybeans R. Bras. Zootec.
Gandra,Jefferson Rodrigues; Takiya,Caio Seiti; Oliveira,Euclides Reuter de; Paiva,Pablo Gomes de; Goes,Rafael Henrique de Tonissi e Buschinelli de; Gandra,Érika Rosendo de Sena; Araki,Hayne Mayumi Cariolano.
ABSTRACT This study was undertaken to determine the effects of chitosan and whole raw soybean on nutrient intake and total tract digestion, nitrogen utilization, microbial protein synthesis, blood metabolites, and energy balance of dairy heifers. Twelve Jersey heifers (6±0.5 months of age and 139.50±25.56 kg of live weight; mean ± standard deviation) were randomly assigned to a replicated Latin square design with a 2 × 2 factorial arrangement. The experimental period consisted of 14 days of adaptation to diets, six days of sampling, and five days of washout. The experimental diets were: control (CO); chitosan (CHI, inclusion of 2.0 g kg−1 DM of chitosan); whole raw soybean (WS, 163.0 g kg−1 of WS on diet DM basis); and chitosan + whole raw soybean...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial; Nitrogen metabolism; Oilseed; Rumen modulator.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016000300130
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Plant biostimulants: physiological responses induced by protein hydrolyzed-based products and humic substances in plant metabolism Scientia Agricola
Nardi,Serenella; Pizzeghello,Diego; Schiavon,Michela; Ertani,Andrea.
ABSTRACT In recent years, the use of biostimulants in sustainable agriculture has been growing. Biostimulants can be obtained from different organic materials and include humic substances (HS), complex organic materials, beneficial chemical elements, peptides and amino acids, inorganic salts, seaweed extracts, chitin and chitosan derivatives, antitranspirants, amino acids and other N-containing substances. The application of biostimulants to plants leads to higher content of nutrients in their tissue and positive metabolic changes. For these reasons, the development of new biostimulants has become a focus of scientific interest. Among their different functions, biostimulants influence plant growth and nitrogen metabolism, especially because of their...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hormones; Nitrogen metabolism; Carbon metabolism; Phenylpropanoid pathway; Stress.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000100018
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Streptococcus mutans GlnK protein: an unusual PII family member BJMBR
Portugal,M.E.G.; Souza,E.M.; Pedrosa,F.O.; Benelli,E.M..
Streptococcus mutans is a Gram-positive bacterium present in the oral cavity, and is considered to be one of the leading causes of dental caries. S. mutans has a glnK gene, which codes for a PII-like protein that is possibly involved in the integration of carbon, nitrogen and energy metabolism in several organisms. To characterize the GlnK protein of S. mutans, the glnK gene was amplified by PCR, and cloned into the expression vectors pET29a(+) and pET28b(+). The native GlnK-Sm was purified by anion exchange (Q-Sepharose) and affinity (Hi-Trap Heparin) chromatography. The GlnK-His-Sm protein was purified using a Hi-Trap Chelating-Ni2+ column. The molecular mass of the GlnK-His-Sm proteins was 85 kDa as determined by gel filtration, indicating that this...
Tipo: Info:eu-repo/semantics/article Palavras-chave: GlnK; Streptococcus mutans; Nitrogen metabolism.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500003
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Variation in the ureide content of Jack Bean during the reproductive stages in response to nitrate BABT
Camargos,Liliane Santos; Aguiar,Leandro Ferreira; Carmezini,José Antônio; Azevedo,Ricardo Antunes.
Nitrogen is frequently the limiting mineral nutrient for plant productivity and it is essential to obtain an understanding of how this element is assimilated and its metabolism regulated, in crop plants. The objective of this work was to study nitrogen metabolism in the tropical legume Canavalia ensiformis, a plant species used as a green manure. The nitrate dose provided changed the concentrations of ureides exported via the xylem, whilst the developmental stage alterations demonstrated to influence the form of ureide exported via the xylem. Considering the content of ureides as an indicator of N-fixation status, it could be concluded that N-fixation was affected in the presence of nitrate in C. ensiformis.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nitrogen metabolism; Allantoin; Allantoic acid; Canavalia ensiformis; Ureides.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300009
Registros recuperados: 9
Primeira ... 1 ... Última
 

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